The Viscum album Gene Space database

نویسندگان

چکیده

The hemiparasitic flowering plant Viscum album (European mistletoe) is known for its very special life cycle, extraordinary biochemical properties, and extremely large genome. size of genome estimated to be 30 times larger than the human 600 model Arabidopsis thaliana . To achieve insights into Gene Space genome, which defined as space including surrounding protein-coding regions, a transcriptome project based on PacBio sequencing has recently been conducted. A database resulting from this contains sequences 39,092 different open reading frames encoding 32,064 distinct proteins. Based ‘Benchmarking Universal Single-Copy Orthologs’ (BUSCO) analysis, completeness was in range 78%. further develop database, we performed V. organs harvested summer winter Illumina sequencing. Data both strategies were combined. new II (VaGs II) 90,039 93% revealed by BUSCO analysis. Sequences other organisms, particularly fungi, are colonize mistletoe leaves, have removed. evaluate quality proteome data mitochondrial fraction re-analyzed. Compared original evaluation published five years ago, nearly 1000 additional proteins could identified fraction, providing Oxidative Phosphorylation System VaGs available at https://viscumalbum.pflanzenproteomik.de/ Furthermore, all uploaded European Nucleotide Archive (ENA).

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ژورنال

عنوان ژورنال: Frontiers in Plant Science

سال: 2023

ISSN: ['1664-462X']

DOI: https://doi.org/10.3389/fpls.2023.1193122